Troubleshooting

Separate Python, external-program, data, network, and scheduler failures.

OrthoEvolution connects Python, external programs, data files, network services, and cluster schedulers. First identify which system stopped. A Python traceback can report a failure that started outside Python.

Confirm the environment

.venv/bin/python --version
.venv/bin/python -c "import OrthoEvol; print(OrthoEvol.__name__)"
.venv/bin/python -m pip check

Use Python 3.11 through 3.14. Run each command with the same local environment that contains OrthoEvolution.

Classify the failure

Boundary First checks
Python import Interpreter path, installed package, dependency conflicts
BLAST Executable on PATH, BLASTDB, database files, accession input
Alignment Executable path, sequence type, file format, writable output
Phylogenetics Program installation, model settings, alignment validity
NCBI retrieval Network access, remote path, storage, archive validation
PBS or Slurm Scheduler command, account, queue or partition, job identifier

Preserve useful evidence

Save the exact command, traceback, log, configuration file, and smallest input that fails. Also record the package, Python, and external-program versions. Before you share the report, remove credentials and private paths.